. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 60 J. M. HUSH AND R. L. OVERALL. CURRENT IN CURRENT OUT WOUND B The effect of wounding the pea root 3 mm back from the tip: current patterns around the wound site (A) 2 min after wounding (mean ± ' <» 1.5 h after wounding (mean ± SEM of 7 roots): (C) 6 i . n ± SEM of 6 roots); (D) 23 h after wounding (mean oots). Diagrammatic representation of the roots as viewed fr. rents are found exclusive ;hc wound area, a dis- tinct peak of 1.2 ^A servable over the wound. Five minutes after wounding ;-splays a dis- tinct asymmetry in
Image details
Contributor:
Library Book Collection / Alamy Stock PhotoImage ID:
RHKJRKFile size:
7.1 MB (105.2 KB Compressed download)Releases:
Model - no | Property - noDo I need a release?Dimensions:
1077 x 2320 px | 18.2 x 39.3 cm | 7.2 x 15.5 inches | 150dpiMore information:
This image is a public domain image, which means either that copyright has expired in the image or the copyright holder has waived their copyright. Alamy charges you a fee for access to the high resolution copy of the image.
This image could have imperfections as it’s either historical or reportage.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 60 J. M. HUSH AND R. L. OVERALL. CURRENT IN CURRENT OUT WOUND B The effect of wounding the pea root 3 mm back from the tip: current patterns around the wound site (A) 2 min after wounding (mean ± ' <» 1.5 h after wounding (mean ± SEM of 7 roots): (C) 6 i . n ± SEM of 6 roots); (D) 23 h after wounding (mean oots). Diagrammatic representation of the roots as viewed fr. rents are found exclusive ;hc wound area, a dis- tinct peak of 1.2 ^A servable over the wound. Five minutes after wounding ;-splays a dis- tinct asymmetry in the pattern scellular ion cur- rents (Fig. 5a). This persists at the wound site even after 1 h (Fig. 5b), although partial symmetry is restored around the rest of the root tip by this time. Ionic composition oj the wound current Table I shows that there is no apparent effect on the wound current, of altering the levels of Na+, K+, Cl~, or H+ in the bathing medium. A very slight wound current enhancement appears to result from reducing external K+ or H+ levels, or increasing Cl~ concentration. How- ever, lowering external Ca2+ (via 1CT4 MEGTA) dramat- ically reduced the current (by about 40%) as did reason- able concentrations of two Ca:+ ion analogues and chan- nel blockers: 100 nAl La3+ cut the current by 50% (Fig. 6) and 50 ^/Gd3+ by about 30%. These results strongly suggest that a Ca2+ current or Ca2+-controlled current may be involved in the wound response. The effects of verapamil, a different type of calcium channel antago- nist, were slight; it is possible that only concentrations higher than the micromolar range employed here, would be effective. Extracellular pH Wounded roots placed on agar plates containing bro- mocresol purple caused a strong pH shift in the agar di- rectly around the wound, as indicated by the appearance of a strong purple band in the agar within a few minutes, which is focussed at the wound site (Fig. 7a). By 20 min the alkaline region is more