Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . The tubes can be placed directly under the microscope forfurther examination of the colonies. Animals as Culture-media.—It isalmost impossible to separate certainorganisms, such as the tubercle bacil-lus and pneumococcus, from mixedcultures by ordinary plate methods,and the plan of producing the diseasein animals by inoculation, and thenobtaining the organism in pure cul-ture, has to be employed. Pure Cultures by Boiling.—Spored organisms may be separatedfrom others by
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Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . The tubes can be placed directly under the microscope forfurther examination of the colonies. Animals as Culture-media.—It isalmost impossible to separate certainorganisms, such as the tubercle bacil-lus and pneumococcus, from mixedcultures by ordinary plate methods, and the plan of producing the diseasein animals by inoculation, and thenobtaining the organism in pure cul-ture, has to be employed. Pure Cultures by Boiling.—Spored organisms may be separatedfrom others by boiling the mixture fora few minutes, when aU the non-sporedforms will perish, and only the sporesremain to germinate subsequently. Fermentation Tube.—For show-ing the presence of gas or fermenta-tion the Smith tube (Fig. 25) or someof its modifications must be used.The closed end and part of the bulbare filled with the glucose or dextrose bouillon and sterilized at low temperatures for three succes-sive days, then inoculated and placed in the incubator. Gasforms gradually, displacing the fluid in the closed end.. Fig. 25.—Smiths fer-mentation tube. 82 ESSENTIALS Or BACTERIOLOGY CHAPTER XII CULTIVATION OF ANAEROBIC BACTERIA Special methods are necessary for the culture of the ana-erobic variety of bacteria in order to procure a space devoidof oxygen. Liboriuss High Cultures.—The tube is filled aboutthree-quarters full with gelatin, which is then steamed in awater-bath and allowed to cool to 40° C, when it is inoculated